发明名称 WATER-SOLUBLE TETRAZOLIUM COMPOUND AND DETERMINATION USING IT
摘要 NEW MATERIAL:A compound shown by the formula I (R<1> is nitrophenyl, or group shown by the formula II; R<4> and R<5> are H, lower alkyl, or R<4> and R<5> form aromatic ring; R<2> is group shown by the formula III; R<6> is H, or COOH; R<7> is H, NO2, CN, halogen, or NR<8>R<9>; R<8> and R<9> are lower alkyl which may have OH; R<3> is polyethylene glycol residue; X is halogen). EXAMPLE:2-( p-Nitrophenyl )-3-{ p-(N,N-diethylamino)phenyl }-5-[p-{hydroxy.poly- (oxy-1,2-ethanediyl)}phenyl]-2H-tetrazolium chloride. USE:A water-soluble reagent useful for determination of a reductive substance, having improved linearity of calibration curve of formed water-soluble formazan. PREPARATION:A reaction product of p-nitrophenylhydrazine and p-R3-substituted benzaldehyde is reacted with a solution of diazotized N,N-diethyl-p-phenylenediamine, prepared formazane is oxidized to give the compound of the example.
申请公布号 JPS6075470(A) 申请公布日期 1985.04.27
申请号 JP19830182497 申请日期 1983.09.30
申请人 WAKO JUNYAKU KOGYO KK 发明人 OGATA HIROSHI;YAMASATO FUJIO;ISHIHARA MASAMI;OOBA HITOSHI;MORII SEIJI;HAYASHIDA KAZUYOSHI
分类号 C07D417/04;C07D257/00;C07D257/04;C07D277/00;C12Q1/26;G01N33/92 主分类号 C07D417/04
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