METHOD FOR THE QUANTITATIVE DETERMINATION OF BILIRUBIN IN BIOLOGICAL FLUIDS

摘要

A novel enzyme of bilirubin oxidase and a conventional enzyme of laccase oxidize bilirubin to biliverdin without formation of hydrogen peroxide. Conjugated bilirubin in biological fluid may be quantitatively determined by reacting therewith the bilirubin oxidase of a microorganism of the genus Myrothecium origin, while total bilirubin in biological fluid may be quantitatively determined by reacting therewith the bilirubin oxidase or laccase under the presence of a specific additive compound.