METHOD FOR PREPARING SAMPLE FOR USE IN ENDOTOXIN TEST AND KIT FOR PREPARING SAID SAMPLE

摘要

A method for preparing a sample for use in endotoxin test comprises subjecting a sample of plasma or blood preparation to a contact with water-insoluble anti human alpha 2-macroglobulin antibody and to a gel filtration by means of a gel of ally dextran cross-linked with N,N min -methylenebis acrylamide or a gel of polyvinyl alcohol having many hydrophilic hydroxyl groups, and recovering the maximum molecular weight fraction. The method can be carried out using a sample preparing kit which consists of a set of (a) a sample-developing column 1 prepared by packing a packing material 2, which is water-insoluble anti human alpha 2-macroglobulin antibody and a gel of ally dextran crosslinked with N,N min -methylenebis acrylamide or a gel of polyvinyl alcohol having many hydrophilic hydroxyl groups, both equilibrated with a buffer solution 3 of pH 7.0-7.4, into a column and tightly sealing the column with a stopper 4 and bottom cap 7, (b) an aqueous solution of a high molecular colored substance to be added to the sample and (c) a buffer solution for use in developing the sample.