| 摘要 |
PURPOSE:To provide a monoclonal antibody recognizing the alpha-fetoprotein existing at the surface of cell membrane, and useful for the remedy of hepatocarcinoma, especially as an antibody for missile therapy. CONSTITUTION:A monoclonal antibody recognizing the alpha-fetoprotein (AFP) exsisting at the surface of cell membrane. In the markers of carcinoma, AFP and fetus antigen (CEA) are well known, and AFP is produced in various hepatocarcinoma and cerulein. Since said monoclonal antibody recognizes the AFP existing at the surface of cell membrane, it is suitable for the missile therapy by bonding the antibody with a carcinostatic agent or a toxin. The antibody can be prepared by (1) immunizing e.g. BALB/C mouse with AFP originated from human placenta, (2) extracting the spleen from the immunized animal, (3) carrying out the fusion with the mouse myeloma cell of e.g. P3-U1 using polyethylene glycol by conventional method to obtain a hybridoma, and (4) separating from the supernatant liquid. The monoclonal antibody prepared by this process is effective to dye the hepatocarcinoma cerulein immunochemically, and to select the positive antibody. |
