| 摘要 |
Disclosed are chromogenic or fluorogenic peptide-type compounds adapted for use in determining endotoxin in a sample by a Limulus amebocyte lysate-type assay. The compounds have the general formula: R1-A1-A2-A3-A4-B-R2 wherein R1 represents hydrogen, a blocking aromatic hydrocarbon or acyl; A1 represents an L or D-amino acid selected from either Ileu, Val or Leu; A2 represents Glu or Asp; A3 represents Ala or Cyst; A4 represents Arg, B represents a linkage group selected from ester and amide linkage groups; and R2 represents a chromogenic or fluorogenic group which is covalently attached to the C-carboxyl terminal of arginine through the B linkage group and which yields a chromophoric or fluorescent marker compound of the formula R2-B-H upon enzymatic hydrolysis from the remainder of the peptide-type compound by activated LAL pro-clot enzyme. Also disclosed are methods for the determination of endotoxin in a sample by contacting the sample with a pro-clotting enzyme from Limulus amebocyte lysate and one of the above chromogenic or fluorogenic peptide-type compounds.
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