发明名称 |
Method and apparatus for cryopreparing biological tissue for ultrastructural analysis |
摘要 |
This invention relates to a method for the cryopreparation of biological tissue samples for ultrastructural analysis. The process comprises vitrifying a biological tissue sample under severely rapid conditions and then depressurized. The depressurized, vitrified tissue sample is brought to equilibrium at a temperature of less than -140 DEG C. The tissue sample is then dehydrated while maintained in a state of thermal equilibrium. After reaching equilibrium the tissue sample is infiltrated with a degassed resin and followed by a polymerization of the resin to form an embedded tissue sample. This invention additionally relates to a sample holder apparatus which has unique use characteristics in the process of this invention.
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申请公布号 |
US4510169(A) |
申请公布日期 |
1985.04.09 |
申请号 |
US19830525626 |
申请日期 |
1983.08.23 |
申请人 |
THE BOARD OF REGENTS, THE UNIVERSITY OF TEXAS |
发明人 |
LINNER, JOHN G. |
分类号 |
G01N1/28;G01N1/30;G01N1/42;G01N33/48;G01N33/96;(IPC1-7):F26B5/06;A01G5/06;A01N1/00;F26B5/10 |
主分类号 |
G01N1/28 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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