ENZYME IMMUNOLOGICAL MEASUREMENT METHOD

摘要

PURPOSE:To make possible the measurement of the immunologically active material in a vital sample with extreme ease and without decreasing the accuracy by combining and using a color forming film which is fixed with a dye in a spot shape so as to correspond to hydrophilic parts. CONSTITUTION:A film carrier disposed with the many hydrophilic parts which can conjugate with the immunologically active material A into the spot shape on the surface of a hydrophobic high-polymer film is used as a solid phase. The color forming film fixed with the dye which is discolored by enzyme only in the positions corresponding to the hydrophilic parts on the film carrier is superposed on the film carrier carrying the labeling immunologically active material. The color forming film is thereby discolored and the color thereof is colorimetrically analyzed. The dye which can be used for the colorimetric quantitative determination of the catalytic activity of the enzyme is exemplified by 5-aminosalicylic acid, etc. These dyes are selected according to the kind of the labeling enzyme; for example, ABTS is adequately selected when the labeling enzyme is peroxidase (HRP).