| 摘要 |
PURPOSE:To enable detection with peptide having about 1,000mol.wt. as well by using the vapor of formaldehyde, polyfunctional aldehyde or polyfunctional isocyanate for fixing reactively the peptide. CONSTITUTION:Isoelectric point electrophoresis using angiotensin II having 1046 dalton mol.wt. as a sample and polyacrylamide gel as a base is performed. The gel of the one-side open type in which polyacrylamide gel is fixed at one side to a glass plate by using methacryloxypropyl methoxy silane is used. The base gel subjected to the isoelectric point electrophoresis is treated with the vapor of gultaraldehyde and the molecules of the separated peptide (angiotensin II) are crosslinked to each other to fix the separated peptide in the base gel. Bi- or more multifunctional aldehydes such as paraformaldehyde and formaldehyde in addition to gluearaldehyde and bi- or more multifunctional isocyanate such as hexamethylene diiosocyanate, tolylene diisocyanate, etc. for isocyanates are usable as the reagent for fixing. |
