METHOD AND COMPOSITION FOR DETERMINING GLYCOSYLATED HEMOGLOBIN

摘要

A process for the determination of glycosilated hemoglobin in a blood sample comprises liberating glycosilated and non-glycosilated hemoglobin from the erythrocytes by chemical or physical treatment; converting, if desired, the hemoglobin into methemoglobin, and thereafter differentiating the glycosilated and non-glycosilated hemoglobin portion using its reaction with haptoglobin, and determining the glycosilated and/or the non-glycosilated hemoglobin portion. The present invention also provides a reagent for the determination of glycosilated hemoglobin in a blood sample, as well as a reagent for the differentiation of glycosilated and non-glycosilated hemoglobin, comprising haptoglobin.