摘要 |
PURPOSE:To provide a novel microbial strain belonging to Nocardia genus and capable of converting a n-paraffin and a fatty acid (derivative) to the corresponding dicarboxylic acid in high efficiency. CONSTITUTION:Nocardia sp. KSM-B-21 (FERM-P No.7006) separated from a source soil using a medium containing n-paraffin by a conventional process. The microbial strain is cultured in a medium containing a n-paraffin or a fatty acid (derivative) as a reaction substrate aerobically at about 28-35 deg.C and about 6.5- 8pH for about 3-5 days. Preferably after separating the microbial cells, the dicarboxylic acid is extracted from the culture liquid with an organic solvent (e.g. mixture of chloroform and methanol) under acidic condition. |