发明名称 |
METHOD FOR THE PREPARATION OF GLUCOSYLTRANSFERASE |
摘要 |
<p>METHOD FOR THE PREPARATION OF GLUCOSYLTRANSFERASE A method of preparing a purified glucosyltransferase (GTF) for use in immunization against dental caries, which method comprises: culturing a Streptococcus mutans in a medium containing glucose and dialyzable nutrients to form a mixture of culture cells and supernatant; recovering the supernatant by the removal of the culture cells; admixing the recovered supernatant with a water-insoluble, polymerized polysaccharide as solid particulate material for a period of time, to provide a GTF, solid particulate complex; recovering the GTF complex in solid particulate form by filtration; washing the solid GTF complex to remove unbound GTF and medium component; removing the GTF from the solid particulate material by a denaturing solvent; recovering the water-insoluble particulate material for reuse in the method; and recovering the GTF from the water-insoluble polysaccharide and purifying the recovered GTF.</p> |
申请公布号 |
CA1183477(A) |
申请公布日期 |
1985.03.05 |
申请号 |
CA19830420426 |
申请日期 |
1983.01.28 |
申请人 |
FORSYTH DENTAL INFIRMARY FOR CHILDREN, 140 FENWAY, BOSTON, MASSACH |
发明人 |
TAUBMAN, MARTIN A.;SMITH, DANIEL J. |
分类号 |
A61K39/09;C12N9/10;C12R1/46;(IPC1-7):C12N9/10 |
主分类号 |
A61K39/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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