EXPRESSIONSPLASMIDER FOER PRODUKTION AV SPECIFIKA PROTEINER.

摘要

A promoter-operator Shine-Dalgarno Clal-containing fragment has been obtained comprising a - 10 region and a Shine-Dalgarno sequence characteristic of the trp promoter-operator Shine-Dalgarno fragment and comprising in addition the following sequences: (i) a 6-base pair sequence in the -35 region of the fragment (5 min ->3 min ) of T/A G/C C/G C/G G/C A/T, and (ii) a sequence of about 17 base pairs between the - 35 and the - 10 regions of said fragment, the sequence of the first eight base pairs (5 min ->3 min ) being C/G G/C C/G G/C C/G A/T T/A C/G. Plasmids have been prepared comprising this promotor-operator fragment and plasmids have also been prepared comprising the trp promoter-operator, which are useful for the over-production in bacteria of particular proteins, such as beta -lactamase, beta -interferon and interleukin 2.