| 摘要 |
PURPOSE:To enable multipication of a bacterium capable of producing industrially an amino acid, nucleic acid, enzyme, antibiotic, etc. by the use of it as a host, by using plasmid pUT32 having a specific molecular weight and a specific scission map of restricted enzyme. CONSTITUTION:Plasmid pUT32 has 4.5 megadalton molecular weight and restricted enzyme map shown by the fig. This plasmid has genetic information with resistance to kanamycin and chloramphenicol, and a large number of copies. Only one position is scissored with restricted enzyme Aua I , BamH I , PvuII, EcoR I , or BglII. A bacterium such as not only Bacillus subtilis K strain, but also industrially useful Bacillus polymyxa, Bacillus amyloliquefacience, Bacillus brevis, etc. belonging to the genus Bacillus can be multiplied as a host, and it is used for multiplication of them. |
