| 摘要 |
PURPOSE:To make it possible to measure the peroxide substance in a small amount of a specimen with high sensitivity, by using bovine serum as a catalyst for use in the quantitative analysis due to chemiluminiscence of the peroxide substance in a living body, and constituting a measuring kit from said serum and an oxidizable luminous reagent. CONSTITUTION:A reagent emitting light upon oxidation, for example, luminol or luciferin is used in measuring a peroxide substance in a living body or processed food. In this case, 1.5-10vol% of bovine serum is added to a reaction system as a catalyst and the pH of the reaction system is pref. adjusted to about 10-12 by a known buffer solution to perform reaction at 0-50 deg.C, pref., 20-37 deg.C. As the specimen, a body fluid component such as blood or a tissue homogenate is used as it is or a composition prepared by dissolving the same in a proper solvent after fractionation or extraction is used. Bovine serum may be added after a liophilized one is rehydrated by distilled water. By this method, a peroxide substance noxious to a living body, especially, peroxide lipid is measured with good activity. |
