| 摘要 |
A method of growing fragile cells, i.e., cells having no cell membrane such as animal cells or genetically modified animal cells, to high density in culture comprises the steps of suspending permeable microcapsules containing a seed culture of such cells in a medium, directly sparging a gas through the medium along the capsules, and allowing the cells to grow. The gas comprises a gas or a selected mixture of gases and is sparged at a rate high enough to attain a substantial partial pressure equilibrium between the gas and liquid phase. The partial pressure of gases essential for cell growth and normal rates of cell mitosis can be maintained in the medium by controlling the composition of the gas and maintaining a threshold sparging level. By sparging, oxygen availability need no longer be a growth-rate limiting factor, and cell densities on the order of 10<8> cells/ml may be achieved. |
