| 摘要 |
PURPOSE:To enable assay of several antigens by using the same water-insoluble carrier insolubilizing the second antibody by binding a complex obtd. by the reaction of antigen with antibody and labelled antibody to a water-insoluble carrier for insolubilizing the second antibody. CONSTITUTION:The assay of antigen in accordance with this invention comprises determination of the amt. of antigen by binding complex obtd. by the reaction between the antigen, antibody (a) and labelled antibody (b) to a water-insoluble carrier for insolubilizing the second antibody, and obtg. the amt. of antigen from the amt. of labelled antibody (b) bonded to the carrier. In this case, in order to permit bonding of the second antibody to the antibody (a) but to prevent from being bonded by the labelled antibody (b), a fact is utilized as one method that separation into bonded part with an antigen, i.e. Fab', Fab from other fragment Fc is possible when an antibody (i.e. immunoglobulin Ig) is decomposed restrictedly using protease such as pepsin or papain, etc. Namely, as antibody, Ig contg. Fc which has not been treated with protease is used; as labelled antiody (b), labelled Fab' or Fab contg. no Fc is used; and as the second antibody, a specific antibody for Fc is used. |
