| 摘要 |
PURPOSE:To impart the ability to produce useful proteins, etc. outside the microbial cells to Escherichia coli, by producing a hybrid plasmid containing a DNA coding a signal amino acid sequence of extracellular proteins in bacteria of the genus Serratia. CONSTITUTION:A plasmid obtained by separating a DNA fragment producing serratia potease SSP-1 from a chromosome DNA of Serratia marcescens IFO3046 which is a bacterium of the genus Serratia, decomposing a commercially available plasmid pBR322 with BamHI on the other hand, treating the resultant decomposed substance with bacterial alkane phosphatase, adding the above- mentioned DNA fragment thereto, adding T4DNA ligase thereto to carry out linking reaction, transforming the Escherichia coli C600 with the resultant linking reaction product, cultivating the transformed microbial cells and isolating the aimed strain capable of secreting and producing the serratia protease SSP-1 outside the microbial cells. |
