| 摘要 |
PURPOSE:To prepare a novel restriction enzyme incision map plasmid having a molecular weight of 3.5M-dalton and containing tetracycline-resistant gene and chloramphenicol-resistant gene. CONSTITUTION:Bacillus subtilis is transformed with the plasmid pTP10 separated from Corynebacterium xerosis, and a plasmid (pTP11) is separated from the transformed cell. The plasmid and the plasmid pNS1 derived from staphylococci are incised with the restriction enzyme HindIII and ligated to obtain the plasmid pTP1102 having a molecular weight of about 4.3M-dalton. The plasmid is incised with the restriction enzyme Xba I , and ligated to obtain the objective plasmid pTP1103 having a molecular weight of about 3.5M-dalton. |
