发明名称 |
Method of culturing anchorage dependent cells |
摘要 |
Disclosed is a method of growing anchorage-dependent cells: cells of the type which normally undergo mitosis only when anchored on a substrate, e.g., fibroblasts or epithelial cells. The method comprises the steps of encapsulating a seed culture of the cells within a semipermeable membrane and suspending the capsules in a growth medium. The interior surfaces of the capsule membrane and/or collagen enclosed within the capsules serve as a substrate for the cells. The ratio of the available substrate surface area to the volume of the culture may be large, thereby allowing the cells to be grown substantially throughout the volume of the culture medium.
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申请公布号 |
US4495288(A) |
申请公布日期 |
1985.01.22 |
申请号 |
US19830484627 |
申请日期 |
1983.04.13 |
申请人 |
DAMON BIOTECH, INC. |
发明人 |
JARVIS, JR., ALLAN P.;LIM, FRANKLIN |
分类号 |
C12N5/00;C12P21/02;(IPC1-7):C12N5/00;C12N5/02;C12N11/00;C12N11/04 |
主分类号 |
C12N5/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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