REAGENT FOR MEASURING LIPASE

摘要

PURPOSE:To enable enzyme immunomeasurement of lipase in a human bodily fluid by forming a sandwich type conjugate of an antibody-antigen labeled antibody and measuring the enzyme activity of the labeled antibody. CONSTITUTION:An antihuman puncreatogenic lipase is manufactured by sensitizing refined human puncreatolipase to a domestic rabbit then the antibody is processed with an insoluble carrier such as a polystyrene bead, glass bead or nylon bead to adsorb the same by covalent bonding or physically on such carrier, by which the insolubilized antibody bound with the antihuman puncreatogenic lipase antibody is obtd. On the other hand, a previously manufactured antihuman puncreatogenic lipase antibody is brought into reaction successively with the compd. optimum for the enzyme used then enzyme to obtain an enzyme labeled antihuman puncreatogenic lipase antibody. The lipase in the patient's serum is measured by using the antihuman puncreatogenic lipase antibody bound carrier and the enzyme labeled antihuman puncreatogenic lipase antibody.