发明名称 |
Immobilized nucleic acid probe and solid support for nucleic acids. |
摘要 |
<p>A solid support capable of binding a nucleic acid thereto upon suitable irradiation, comprising (a) a solid substrate, (b) a photochemically reactive intercalator compound or other nucleic acid-binding ligands, and (c) divalent radical chemically linking the substrate and the ligand (b). Specifically, a hydroxy group-containing solid substrate such as nitrocellulose paper is linked via a bifunctional reagent such as cyanogen bromide or 1,4-butanediol-diglycidyl ether to an amino-substituted angelicin or psoralen or ethidium bromide which in turn is photochemically linked to a nucleic acid. The resulting immobilized nucleic acid probe is capable of hybridizing with complementary nucleic acid fragments and is thereby useful in diagnostic assays.</p> |
申请公布号 |
EP0130523(A2) |
申请公布日期 |
1985.01.09 |
申请号 |
EP19840107266 |
申请日期 |
1984.06.25 |
申请人 |
MOLECULAR DIAGNOSTICS, INC. |
发明人 |
DATTAGUPTA, NANIBHUSHAN;CROTHERS, DONALD M. |
分类号 |
C09H1/00;C07H21/00;C07H21/04;C08H1/00;C12Q1/68;G01N33/53;G01N33/547;G01N33/548;(IPC1-7):C12Q1/68 |
主分类号 |
C09H1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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