Anti-haemophilia factor concentrate prodn.

摘要

Prodn. of antihaemophilia factor (AHF) concentrate comprises (1) purifying an aq. soln of AHF proteins; (2) ultrafiltration of the purified soln. to remove water, then (3) mixing the concentrate with buffer and saline. The resulting soln. is then opt. freeze-dried. - Pref. purification is by mixing with aluminium hydroxide adsorbent and ultrafiltration is through a membrane having a nominal mol wt. cut-off 10000-300000; particularly a hollow-fibre membrane. After ultrafiltration, the soln. can be purified further by adding glycine (before mixing with buffer and saline). Ultrafiltration is carried out without loss of AHF activity (contrast conventional reprecipitation-ultrafiltration) and the prod. has a significantly higher content of von Willebrandt factor which also has a longer invivo half-life. The ratio of AHF:fibrinogen is improved, about 3:1, so the danger of fibrinogen overload is avoided. EPAB- EP-126789 B A process for the production of antihemophilic factor concentrate, characterised in that it comprises the steps of (a) subjecting an aqueous solution of an antihemophilic blood plasma cryoprecipitate to purification by mixing with an aluminium hydroxide absorbent, (b) subjecting the aqueous solution to ultrafiltration using an ultrafiltration membrane having a nominal molecular weight cut-off within the range of 10,000 to 300,000 daltons, (c) mixing the ultrafiltered aqueous solution with glycine, (d) dissolving the paste obtained in step (c) and mixing the solution with buffer and saline, and (e) obtionally freeze-drying the ultrafiltered aqueous solution of step (d). (10pp) - EP-126789 B A process for the production of antihemophilic factor concentrate, characterised in that it comprises the steps of (a) subjecting an aqueous solution of an antihemophilic blood plasma cryoprecipitate to purification by mixing with an aluminium hydroxide absorbent, (b) subjecting the aqueous solution to ultrafiltration using an ultrafiltration membrane having a nominal molecular weight cut-off within the range of 10,000 to 300,000 daltons, (c) mixing the ultrafiltered aqueous solution with glycine, (d) dissolving the paste obtained in step (c) and mixing the solution with buffer and saline, and (e) optionally freeze-drying the ultrafiltered aqueous solution of step (d).