| 摘要 |
PURPOSE:To measure the density of neutral lipid contained in blood serum etc. at a high accuracy by using lipoprotein lipase and glycerol oxidase as immobilized enzyme. CONSTITUTION:A teflon membrane 4 which transmits oxygen molecules is installed to the bottom of the outer tube 2 of a neutral lipid sensor, and outside the teflon memberane, a neutral lipid transmission membrane 7 containing immobilized engyme mixed with lipoprotein lipase and glycerol oxidase is provided. This neutral lipid sensor is immersed in a solution and blood serum specimen is injected, then neutral lipid contained in the blood serum reacts with water in the solution with lipoprotein lipase in the immobilized enzyme used as a catalyst. By this reaction, glycerose and fatty acid are produced, and glycerose reacts with oxygen in the solution with glycerol oxidase in the immobilized enzyme used as a catalyst and produces glyceryl aldehyde and hydrogen peroxide. As a result of this, a current between a cathode 3 and an anode 2 decreases and neutral lipid can be measured. |
