| 摘要 |
PURPOSE:To enable simultaneously measurement reaction of enzymatic activity and condensation reaction, and to make measurement possible either by rate assay method or by end point method, by condensing a color developing agent with a coupler in the presence of bilirubin oxidase or laccase. CONSTITUTION:For example, 0.1M phosphate buffer solution containing 1mM L- leucyl-p-diethylaminoanilide, 0.5mM calcium 1-naphthol-2-sulfonate, and 10mu/ml bulirubin oxidase is prepared. 1ml of this buffer solution is blended with 0-8mul standard serum containing 155mu/ml leucine aminopeptidase, and reacted at 37 deg.C, so that it is colored. The absorbance of coloring is measured at 670nm, and the relationship between leucine aminopeptidase activity (u/ml) and absorbance (OD/ min) is shown in a straight line. Consequently, leucine aminopeptidase can be measured.
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