Expression of foreign genes in yeast.

摘要

A method of expressing foreign genes in yeast comprising transformation of yeast cells by introduction therein of a cloning vector containing a foreign gene, and yeast galactokinase gene regulatory region and promoter in position to regulate the expression of the foreign gene, and cultivating said transformed yeast cells in a nutrient medium under protein-producing conditions is disclosed. Suitable plasmids and microorganisms useful in the process are also disclosed. The method has been used to produce a GAL 1 - bovine calf prochymosin fusion protein in Saccharomyces cerevisiae.