A STABILIZED AQUEOUS COENZYME SOLUTION FOR USE IN A CLINICAL ASSAY, A METHOD OF STABILIZING A LABILE COENZYME IN AN AQUEOUS CLINICAL ASSAY SOLUTION AND A KIT FOR USE IN SAID CLINICAL ASSAY

摘要

A stablized aqueous coenzyme solution for use in the clinical assay of a selected biological constituent through the enzymic reaction of an enzyme with a selective substrate for the enzyme and a coenzyme. The aqueous coenzyme solution comprises at least one first enzyme and a first substrate for selectively reacting with the first enzyme in the assay, a coenzyme for interacting with the first enzyme and first substrate in the assay which coenzyme degrades forming a coenzyme conversion product, and an enzyme and substrate system for regenerating the coenzyme from the coenzyme conversion product. The enzyme and substrate system comprises at least one second enzyme and second substrate selective for the second enzyme which react with the coenzyme conversion product to regenerate the coenzyme. A kit for performing the diagnostic assay is also disclosed.