METHOD OF IMMOBILIZING AN ENZYME IN A BUNDLE OF CELLULOSIC FIBRES FOR A FIBRE-BUNDLE DIALYZER FOR PURIFYING BLOOD

摘要

The object of the method is to immobilize a desired enzyme on the lumen surfaces of the dialyzer fibres typically having an inner diameter of 200-300 microns and wall thickness of 10-20 microns, without prejudice to the chemicophysical, dialyzing and bio-compatibility properties of the fibres.
The enzyme is anchored directly or indirectly through covalent bonds upon activation of the lumen surfaces by circulation through the lumens of an aqueous solution of sodium periodate at a concentration of 0.7 - 21 mg/ml, at a circulation rate of 300-500 mm<3>/minute referred to 1 mm<2> of cross-sectional area of the lumen, at 15-30 DEG C for a time not exceeding 70 minutes.