| 摘要 |
PURPOSE:To enable the continuous industrial use of yeast, by culturing an agglutinating yeast in the presence of a carrier composed of a water-insoluble polyvinyl formal having three-dimensional network open-cell structure, thereby caus- ing the agglutination of the proliferated yeast cells and the entrapping of the agglutinated cells in the pores of the carrier. CONSTITUTION:An agglutinating ethanol-fermentation yeast is cultured in the presence of a carrier consisting of the sheet, cut pieces, or granules of a water- insoluble polyvinyl formal which is a polyvinyl formal resin having a degree of formalization of about 80-86% and having a three-dimensional network open- cell structure with an average pore diameter of about 30-700mu. The proliferated yeast cells are agglutinated and entrapped biologically in the pore of the carrier. A liquid containing an ethanol fermentation sugar selected from sugar syrup, saccharified starch syrup or saccharified cellulose syrup is made to contact with said immobilized yeast. Since the resin has large pores, the substrate solution can be continuously passed through the carrier without causing the clogging, and accordingly, a continuous operation can be carried out by the use of the above immobilized yeast.
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