| 摘要 |
NEW MATERIAL:A plasmid pSAN483, having 5.00+ or -0.10 megadalton (hereinafter abbreviated to MD) molecular weight and one cleaved position (hereinafter abbreviated to site) by restriction enzymes XhoI and PstI and two sites by restriction enzymes MluI, BglII and SphI, the sites being as follows based on the site cleaved by XhoI as the coordinate point; PstI at 0.02MD, MluI sites at 0.37MD and 0.99MD, BglII sites at 1.85MD and 3.28MD and SphI sites at 0.01MD and 4.41MD and shown in the figure. USE:A recombinant DNA and cloned vector of exogenotes. PREPARATION:Streptomyces sp. SANK 60985 strain is cultivated to give mycelia, which are then subjected to bacteriolysis. The resultant bacteriolysis product is then extracted and subjected to equilibrium density gadent centrifugation to afford a mixture of a plasmid pSAN483 with a plasmid pSAN484, which is further treated with a restriction enzyme, mixed with cesium chloride and EtBr, subjected to equilibrium density gradient centrifugation, extracted and dialyzed to isolate the aimed plasmid pSAN483. |
