| 摘要 |
PURPOSE:To obtain enzyme labeled oxy-toxin for enabling highly sensitive quantitative analysis of oxy-toxin, by reacting a specific chemical coupling agent and oxy-toxin while bonding specific enzyme to the resulting reaction product. CONSTITUTION:A coupling agent represented by formula I (wherein X is a functional group capable of forming a sulfide bond or a disulfide bond upon reaction with SH of protein, A is a lower alkylene group and Y is COOH or a reactive derivative thereof), for example, a chemical coupling agent selected from compounds shown by formulae II, III is reacted with oxy-toxin (parts posterior hypophysis hormone), if necessary, in the presence of a condensing agent such as water-soluble carbodiimide to form a composite. beta-D-galactositase is bonded to this composite to obtain enzyme labeled oxy- toxin. On the other hand, a rabbit is immunized with a composite obtained by bonding oxy-toxin to albumin to obtain anti-serum. An oxy-toxin-containing specimen, enzyme labeled oxy-toxin and anti-serum are reacted while the formed precipitate is added to a substrate to measure enzymatic activity. This measured value is compared with a caliburation curve preliminarily calculated from known oxy-toxin specimens to enable the quantitative analysis of oxy-toxin in a specimen with good accuracy. |
