摘要 |
PURPOSE:To obtain a triglyceride sensor which permits continuous measurement by providing a high polymer film immobilized with an enzyme for hydrolysing glycerol ester on a gate insulating film of a pH-sensitive ion-selective field effect transistor. CONSTITUTION:A pH-sensitive ion-selective field effect transistor (TR)1 is a long sized TR provided with a gate part 2 at one end and drain and source contacts 3, 4 at the other end. The gate part 2 is formed by forming a drain diffused region 6 and source diffused regions 7, 8 on a silicon substrate 5 and covering successively the entire part with an oxidized film 9 and a silicon nitride film 10. The surface of the film 10 is chemically modified with gamma-aminopropyl triethoxysilane to introduce a functional group therein and thereafter a porous high polymer film of triacetyl cellulose-triamine-glutaraldehyde is formed thereon. Said film is treated with glutaraldehyde and is brought into reaction with an enzyme soln. to immobilize the enzyme. |