MODEL MOUSE, METHOD OF CONSTRUCTING THE SAME AND USE THEREOF

摘要

It is intended to provide a model mouse which is to be used for clarifying the functions of Gsdmd on intestinal epithelial cells and Gsdmd gene encoding the same. A mouse having a homozygote in which Gsdmd gene encoding Gsdmd has been knock-out on chromosome and not expressing Gsdmd is referred to as the model mouse. First, a targeting vector having a fragment which contains the exon 2 of Gsdmd gene in mouse genomic DNA is transferred into mouse embryonic stem cells and chimeric embryonic cells are constructed from the embryonic stem cells having the Gsdmd gene knock-out by homologous recombination. Next, these chimeric cells are transplanted into a host mouse. The Gsdmd gene-knock out chimeric mice thus bred are mated and heterozygous mice are selected. By mating these heterozygous mice with each other, a model mouse of the Gsdmd gene-knockout homozygote can be obtained.