LIPOSOME MEDIATED TRANSFORMATION OF EUKARYOTIC CELLS

摘要

A process for delivering a substance past the cell membrane of a cell comprising: (a) supplying an appropriate lipid mixture; (b) dissolving said lipid mixture in a solvent miscible with water; (c) forming a lipid-solvent-aqueous preparation by adding to said dissolved lipid-solvent mixture an aqueous solution of said substance; (d) intimately mixing said dissolved lipid-solvent-aqueous preparation by means non- destructive to said substance; (e) removing said solvent to form liposomes; and (g) contacting said cell with said liposomes in a suitable buffer. Plant cell protoplasts are transformed with DNA.