RADIOIMMUNOASSAY OF MIGRATION INHIBITORY FACTOR

摘要

A method is disclosed for detecting the presence of migration inhibitory factor (MIF) which comprises reacting a test solution containing an unknown amount of MIF antigen, a known amount of MIF antigen which has been radiolabeled, a known amount of antibodies to MIF, and thereafter precipitating and removing the resulting immune complex and measuring the radioactive content thereof, said antibodies to MIF being produced by immunization of animals with MIF antigen obtained from the growth of human lymphocytes under tissue culture conditions.