METHODS FOR GENERATING AUGMENTED STEM CELL-DERIVED β CELLS AND USES THEREOF

摘要

Disclosed herein are methods for generating augmented SC-P cells, and isolated populations of augmented SC-P cells for use in various applications, such as cell therapy. There is a need for methods of generating augmented stem cell-derived P (SC-P) cells. The present invention is directed toward solutions to address this need, in addition to having other desirable characteristics. In accordance with an embodiment of the present invention, a method for generating augmented stem cell-derived P (SC-3) cells is provided. The method includes contacting a cell population comprising SC-P cells, or precursors thereof, with an effective amount of an agent that decreases the level and/or activity of AXL receptor tyrosine kinase (AXL) and an antioxidant for a period of time sufficient for the level of MAFA gene expression to increase in the SC-P cells to at least 2 fold greater than the level of MAFA gene expression in the SC-P cells in the absence of contact with the agent and the antioxidant, thereby generating augmented SC-P cells.