| 摘要 |
<p>A method of determining whether two organisms are identical comprises subjecting a restriction endonuclease digest of genomic DNA of the first organism to electrophoresis; determining the positions of the DNA fragments thus-separated which hybridise with one or more labelled DNA probes; and comparing these positions with the positions of DNA fragments binding to the or each probe and produced from genomic DNA of the second organism in like manner; an amount of probe DNA and one or more restriction endonucleases being used such that sufficient bands are revealed by the probing to achieve a sufficiently low probability (X) that the two organismswill have failed to have been distinguished when they appear identical from their band patterns, as determined by: X = F<q> wherein F is a fraction representative of the proportion DNA fragments which are identical between restriction endonuclease digests of genomic DNA of pairs of independently-obtained organisms of the same species as the first and second organisms and q is the number of positions revealed by the probing.</p> |
