Method of measuring biological ligands.

摘要

A highly sensitive and specific method of measuring a biological ligand suitable as a clinical test for the determination of physiological substances and trace components in humoral fluid comprises allowing there to coexist in an aqueous medium a biologically active composition comprising an immobilised antibody phase or immobilised ligand phase on the one hand and an immobilised biotinyl enzyme or biotinyl enzyme inhibitor phase on the other hand, a water-soluble material combination of those of the ligand or the antibody on the one hand and the biotinyl enzyme inhibitor or the biotinyl enzyme on the other hand which are absent from the biologically active composition, and the ligand to be measured and measuring the residual biotinyl enzyme activity or the biotinyl enzyme inhibitor activity and utilising the results as a measure of the ligand to be measured.