| 摘要 |
PURPOSE:To obtain magnetic particles suitable for immobilizing of a living body component by adjusting a soln. mixture contg. gelatin, water soluble polysaccharide, metaphosphate and pulverous ferromagnetic material particles in a specific pH range then forming the insoluble particles within a specific grain size range by an aldehyde crosslinking agent. CONSTITUTION:Pulverous ferromagnetic material particles are dispersed in an aq. soln. contg. sodium metaphosphate and an anionic surface active agent. The dispersion is added to the mixed soln. obtd. by adding an aq. soln. of polysoccharide such as gum arabic removed of insoluble matter to an aq. soln. of gelatin adjusted with pH 9 and further mixing an aq. soln. of C2H5OH. The gelatin soln. dispersed therein with the magnetic particle is adjusted to 2.5-6pH with an aq. acetic acid and after the soln. is cooled to about 5 deg.C, a crosslinking agent such as glutaraldehyde or the like is added under stirring thereby obtaining hydrophilic and insoluble particles having 1-100mu grain size are obtd. Such particles are utilized for a solid carrier, etc. which permit easy operation such as washing and separation by taking advantage of the magnetic characteristic when a living component such as antigen, antibody or the like is going to be immobilized. |
