| 摘要 |
A process and apparatus for the multiplexed detection of nucleic acid targets directly from samples containing blood by quantitative real-time PCR means. The process comprises the addition of blood directly into the PCR reaction, including the nucleic acid of the target species, and an optical interrogation method centred on illumination at wavelengths exceeding 620nm. Preferably the illumination is provided by red 635nm laser diodes delivering excitation power in excess of 2mW and delivered to the sample by optic fiber, further that spectrophotometry is utilised to make possible multiplexed detection of targets in the presence of high amounts of blood. Such a process and apparatus is capable of overcoming the >90% inhibition of fluorescence that the presence of blood would normally present, rendering QPCR possible. |
