摘要 |
NEW MATERIAL:Bacillus licheniformis MN001 having the following mycologic properties. (A) Shape: a bacillus having 0.5-0.8mum width and 2-3mum length; positive in gram stain; forming elliptic endospore; having motion properties. (B) Growth: growing extremely well in a common agar medium, not forming dyestuff; growing extremely well in a medium consisting essentially of molasses and ammonium sulfate, not forming dyestuff; growing in any of 5, 7, and 10wt% salt solution medium. (C) Physiological properties; + in catalase formation, + in growth under anaerobic condition, + in VP reaction, + in high-temperature growth ability at 50 deg.C, and - at 55 deg.C. Others are shown by the table. USE:An animal feed or additive for feed. PREPARATION:The titled bacterium is cultivated by liquid or solid culture by a common fermentation method. The composition of the medium: a carbon source is glucose, starch, mollasses, etc.; a nitrogen source is peptone, meat extract, soybean cake, urea, etc.; magnesium, etc. is used as an inorganic source. The prepared culture is directly processed or washed and processed into only a mold, which is lyophilized or subjected to spray drying to give a pharmaceutical preparation. |