| 摘要 |
PURPOSE:To obtain an immobilized lipase stable for a long period (at 0-60 deg.C) and having high activity, by adsorbinh a lipase originated from Candida cylindracea to a carrier comprising a specific macroporous anion exchange resin, and reacting the product with a polyfunctional crosslinking agent. CONSTITUTION:A lipase originated from Candida cylindracea is adsorbed to a carrier comprising a macroporous phenol-formaldehyde anion exchange resin having a specific surface area of >=5m<2>/g, the total volume of the macropores having a pore diameter of 1,000-2,000Angstrom of >=0.2ml/g, and an anion exchange capacity of (substituted) amino group of >=1meg/g, preferably at 4.5-7.5pH for 1-15hr. The adsorbed product is made to react with a polyfunctional crosslinking agent (preferably a polyaldehyde such as glyoxal) at a concentration of preferably 0.2-2W/V%, 4-7pH and 15-35 deg.C for 0.5-20hr to obtain the objective immobilized lipase.
|
