Process for determining enzymatic activity.

摘要

This invention provides a process for determining the activity of an object enzyme in a chain reaction system consisting of an object enzyme and a conjugate enzyme, which comprises determining the maximum reaction velocity of the conjugate enzyme (Vmax; 2) and dividing the reaction velocity of the conjugate enzyme (V2) by the reaction velocity ratio (r2; reaction velocity of conjugate enzyme/reaction velocity of object enzyme) which is determined by substituting (Vmax; 2) in the following equation (6) <IMAGE> or diluting the conjugate enzyme to prepare at least two solutions of different concentrations (nothing but the concentration ratio is known), determining V2 at each concentration and dividing the resulting V2 by r2 determined by plotting. This invention provides a process for determining the activity of an object enzyme accurately to thereby make it possible to prepare a diagnostic determining kit.