| 摘要 |
PURPOSE:To maintain stably enzyme activity for a long time in a measuring device for a pyruvic acid using immobilized pyruvic acid oxidase by bringing a specific concn. range of flavin adenine dinucleotide (FAD) soln. into the immobilized oxidase. CONSTITUTION:An FAD soln. is supplied through a piping 10 having a small inside diameter at a measured supplying rate of 10<-9>-10<-6>mol/min from a storage tank 12 by using a micro metering pump to an electrode part 15 of a measuring device for a pyruvic acid using a film 6 formed by fixing pyruvic acid oxidase (POP) to collagen, polyacrylamide, etc. A sample is supplied from a supplying part 14 and a buffer or substrate soln. is supplied from a storage tank 11, respectively to the piping 9 of the part 15. The FAD soln. is brought into the POP immobilized film 16 through the ultrafilter film 2 and buffer layer 3 of the part 15 in the above-mentioned way, by which the activity of POP is maintained stably for a long time. The same result is obtainable by bringing the FAD soln. into contact with said film in the stage of non-measuring or washing the part 15 in place of doing the same in the stage of measurement. |
