摘要 |
PURPOSE:A microorganism in Pseudomonas, capalbe of producing methyl formate, is cultured in a medium containing methanol as a carbon source to produce methyl formate. CONSTITUTION:Novel strains, Pseudomonas extorquens K-1345 and K-1338-1 have beeen isolated from soil. The microorganism is inoculated to a medium containing methanol, as a carbon source, an organic ammonium salt, inorganic ammonium salt or urea as a nitrogen source. Then, aerobic cultivation is effected at 20-40 deg.C for 12hr to 10 days to produce methyl formate. Static culture may be carried out in the latter half of the cultivation.
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