Protective solution for preserving functional cells

摘要

A protective solution for the preservation of functional cells containing saccharose, and a phosphates buffer having a high concentration. The osmolarity of the solution is 330+/-10 mOsm and its pH 7.3+/-0.2. It can be mixed directly with red corpuscles of human or animal origin, with other blood cells such as leucocytes and platelets, and with cellular suspensions from various organs, either for their preservation for a long time, of the order of one week, in situ after thawing, or during transportation. The preferred composition is -Adenine 0.085 g +/- 0.010 -Glucose 5 g +/- 0.5 -Saccharose 47 g +/- 2 -Monosodium phosphate monohydrate 0.75 g +/- 0.08 -Disodium phosphate dihydrate 4 g +/- 0.5 -Sodium chloride 2.5 g +/- 0.5 -Distilled water qsp 1000 ml -