| 摘要 |
PURPOSE:To prepare a physiologically active substance, mutastein, useful for the prevention and remedy of dental caries, by culturing microorganism belonging to Cercospora genus, Cladosporium genus, Collectotrichum genus, etc. under aerobic condition, and extracting the cultured liquid. CONSTITUTION:Microorganism capable of producing mutastein and belonging to Cercospora genus, Cladosporium genus, Colletotrichum genus, Cylindrosporium genus, Dactylaria genus, Dematium genus, Diplogelasinospora genus, Drechslera genus, Endophragmia genus, Fennellia genus, Leptographium genus, Monascus genus, Mucor genus, Doratomyces genus, Sporormiella genus or Stachybotrys genus, is cultured aerobically at 20-30 deg.C, and the objective physiologically active substance, mutastein, is separated from the culture liquid by salting-out with ammonium sulfate, pH-precipitation, ion exchange chromatography, etc. |
