Methods and structures employing non-radioactive chemically-labeled polynucleotide probes.

摘要

Non-radioactive chemically-labeled polynucleotide probes, such as non-radioactive chemically-labeled, single- stranded DNA probes, are hybridized to fixed, denatured, single-stranded genetic material having a make-up complementary to said probes for determining the identification and/or presence of said genetic material. The non-radioactive chemical label on said probe is employed to elicit a response therefrom so as to determine the identity and/or presence of said genetic material. It is preferred that the non-radioactive chemical label attached to the probe have an enzyme attached thereto after hybridization, and that the presence of the enzyme attached to the chemically-labeled probe be elicited by spectro-photometric assay techniques.