| 摘要 |
PURPOSE:To obtain an anti-inflammatory agent having high safety and activity, easily, by extracting and purifying a thiol protease inhibitor from human urine using a combination of two or more extraction means such as molecular filter, ion exchange material, adsorbent, etc., and using the purified substance as an active component. CONSTITUTION:A thiol protease inhibitor is extracted and purified from human urine by a combination of two or more extraction means selected from molecular filter (e.g. sephadex), ion exchange material (e.g. DEAE-cellulose), adsorbent (e.g. hydroxyapatide) and affinity chromatography (e.g. trypsine-sephalose). The obtaind inhibitor improves the inflammation caused by thiol protease, and is also useful as a remedy for muscular dystrophy and renal hypertension in which thiol protease is participated. The extraction and purification can be carried out by a relatively simple process, and the method can be applied to mass production. There is no problem of antigenicity.
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