THE CLONING AND EXPRESSION OF NUCLEOTIDE SEQUENCES ENCODING BOVINE GROWTH HORMONE

摘要

Methods and compositions are provided for the cloning and expression of bovine growth hormone (BGH) genetic sequences in single-cell host organisms. Also described are methods for culturing these novel single cell organisms to produce polypeptides having growth hormone activity and methods for purification of the gene product. Recombinant plasmids were constructed so as to provide an "optimal" system for the expression of BGH gene sequences in compatible host cells. The BGH related proteins produced by the recombinant DNA techniques described herein may be used in animal husbandry and in veterinary medicine.