摘要 |
A medium for the selective growth and identification of Streptococcus mutans bacteria is disclosed, that includes a tryptone-glucose extract agar, monobasic and dibasic potassium phosphate, yeast extract, agar, a color indicator for the bacteria and a solution of sucrose ranging in concentration from 1% to 15%. Preferably, the concentration of the sucrose may range from about 3% to about 11%. A bacteria culture plate may be prepared comprising the medium, and may include a first basal layer comprised entirely of the medium, and a second overlayer agar coating, including a mixture of the medium with a calcium phosphate suspension. Both the basal layer and the overlayer agar coating are preferably adjusted to a mildly basic pH. The present medium, and bacteria culture plates prepared therewith, offer desired bacterial specificity with no growth inhibition, that is usually the case with specific media of this kind. The present medium and bacteria culture plate is particularly useful, as Streptococcus mutans is considered to be instrumental in dental caries development.
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